rabbit polyclonal anti-brain-derived neurotrophic factor  (Merck KGaA)

Journal: International Journal of Molecular Sciences

Article Title: Sex-Dependent Effects of Chronic Restraint Stress on Mood-Related Behaviours and Neurochemistry in Mice

doi: 10.3390/ijms241210353

Figure Lengend Snippet: Specificity, species, manufacturer, and dilution details of the antibodies used.

Article Snippet: Anti-mature brain-derived neurotrophic factor (mBDNF) , Polyclonal Rabbit , Osenses (Cat# 2728, RRID: Not available) , 1:4000 in 5% BSA/TBST.

Techniques:

Journal: Nutrients

Article Title: Long-Term Diet Supplementation with Lactobacillus paracasei K71 Prevents Age-Related Cognitive Decline in Senescence-Accelerated Mouse Prone 8

doi: 10.3390/nu10060762

Figure Lengend Snippet: Effects of 43-week Lactobacillus strain administration on the expression levels of brain-derived neurotrophic factor (BDNF) in the hippocampus and cortex of SAMP8. ( A ) Bdnf mRNA levels measured by quantitative PCR ( n = 8 mice per group); ( B ) Western blotting analysis ( n = 4 mice per group) of the protein levels of BDNF; ( C ) Quantification of band intensities in ( B ). Data are expressed as mean ± SEM, * p < 0.05 vs. the control group.

Article Snippet: The membranes were blocked with 3% BSA in Tris-buffered saline with 0.05% Tween-20 for 1 h at room temperature, and incubated overnight at 4 °C with the following antibodies: rabbit polyclonal anti-brain-derived neurotrophic factor (BDNF; 1:3000; Abcam, Cambridge, MA, USA), rabbit monoclonal anti-cAMP response element binding protein (CREB; 1:2000; Abcam), rabbit monoclonal anti-Ser133-phosphorylated CREB (pCREB; 1:2000; Abcam), and mouse monoclonal anti-β-actin (1:5000; Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Expressing, Derivative Assay, Real-time Polymerase Chain Reaction, Western Blot

Journal: Nutrients

Article Title: Long-Term Diet Supplementation with Lactobacillus paracasei K71 Prevents Age-Related Cognitive Decline in Senescence-Accelerated Mouse Prone 8

doi: 10.3390/nu10060762

Figure Lengend Snippet: Representative images of BDNF immunofluorescence (green) in the hippocampal dentate gyrus (DG) and CA3 regions of SAMP8. DAPI stained nuclei (blue). Scale bar: 400 μm.

Article Snippet: The membranes were blocked with 3% BSA in Tris-buffered saline with 0.05% Tween-20 for 1 h at room temperature, and incubated overnight at 4 °C with the following antibodies: rabbit polyclonal anti-brain-derived neurotrophic factor (BDNF; 1:3000; Abcam, Cambridge, MA, USA), rabbit monoclonal anti-cAMP response element binding protein (CREB; 1:2000; Abcam), rabbit monoclonal anti-Ser133-phosphorylated CREB (pCREB; 1:2000; Abcam), and mouse monoclonal anti-β-actin (1:5000; Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Immunofluorescence, Staining